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• The highest transfection efficiency in many cell types and formats (e.g. 96-well). Refer to the Transfection Collection and the Invitrogen Transfection Guide available at www.invitrogen.com for a list of cell lines and cell types that have been successfully transfected. Detailed transfection procedures are also available. For a procedure to transfect mammalian cells with short interfering RNAs (siRNA) for use in RNA interference (RNAi) studies, see www.invitrogen.com\rnai.
• DNA-Lipofectamine™ 2000 complexes can be added directly to cells in culture medium (in the presence or absence of serum).
• It is not necessary to remove complexes or change or add medium following transfection, although complexes can be removed after 4-6 hours without loss of activity.